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AN EFFICIENT PROTOCOL FOR IN VITRO PROPAGATION OF CARNATION (DIANTHUS CARYOPHYLLUS)

AAMIR ALI1, HUMERA AFRASIAB2, SHAGUFTA NAZ3, MAMOONA RAUF2 AND JAVED IQBAL2 

Abstract: The present research work involves shoot formation, their multiplication and rooting in carnation Dianthus caryophyllous. For shoot formation both apical and nodal meristems were used. MS medium containing BAP alone or in combination with kinetin was tested. Best shoot formation response was obtained after 6 days of inoculation from apical meristem and after 7 days of inoculation from nodal meristem on MS medium supplemented with 4.0 mg/l BAP. Apical meristem showed more pronounced effect for shoot formation than nodal meristem. Well-developed shoots were shifted for their multiplication. Maximum number of multiple shoots were obtained on MS medium containing 1.0 mg/l BAP. These multiple shoots increased in their number when were given subsequent incubation period. Addition of Kinetin to BAP failed to show good shoot multiplication response. Shoots after attaining the size of 5.0 cm were shifted for rooting. Best rooting response was obtained on MS medium containing 1.0 mg/l NAA. Well rooted plants were shifted into glass house for hardening and acclimatization and were shifted to natural climatic conditions.

1Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan.

2Seed Center, University of the Punjab, Quaid-e-Azam Campus, 54590, Lahore, Pakistan

3Department of Botany, Lahore College for Women University, Lahore, Pakistan