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IN VITRO REGENERATION OF PLANTLETS FROM SEEDLINGS EXPLANTS OF GUAVA (PSIDIUM GUAJAVA L.) CV. SAFEDA

S. TARIQ SHAH¹, ROSHAN ZAMIR¹, J. AHMAD², HAIDAR ALI³ AND G. LUTFULLAH²

Abstract: Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from In vitro germinated (50 days-old) seedlings of Psidium guajava L. Cv. Safeda. Modified Murashige & Skoog (MMS) medium supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberelic acid (GA3), Zeatin 1.0 mg/L combined with GA3 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted on ½ strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L, where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.

¹Nuclear Institute for Food and Agriculture Peshawar, Pakistan

²Centre of Biotechnology, University of Peshawar, Peshawar, Pakistan

³Department of Botany, University of Karachi, Karachi-75270, Pakistan