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COMPARISON OF METHODS OF INOCULATION OF XANTHOMONAS ORYZAE PV.ORYZAE IN RICE CULTIVARS

M.A. AKHTAR¹, ABDUL RAFI² AND ABDUL HAMEED³

Abstract: Bacterial suspension containing 10⁸ colony forming units (cfu) of Xanthomonas oryzae pv. oryzae was used for inoculating detached leaves of 6 commercial rice cultivars In vitro as well as for testing these cultivars in pots in growth chamber conditions by three methods of inoculation i.e., clipping, pin prick and brush respectively. Clipping method resulted in much more lesion length development than either the pin prick and brush method.

¹Crop Diseases Research Programme, Institute of Plant and Environment Protection, National Agricultural Research Centre, Islamabad, Pakistan

²Department of Plant Pathology, NWFP Agricultural University, Peshawar, Pakistan

³Department of Biological Sciences, Quaid-e-Azam University, Islamabad, Pakistan.