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ANTIBACTERIAL ACTIVITIES OF HONEY, SANDAL OIL AND BLACK PEPPER

IFRA GHORI AND SHEIKH SAEED AHMAD^*

Abstract: The purpose of the present study was to determine the antibacterial activities of honey, sandal oil and black pepper by taking a selected standard medicine. These products are used for the treatment of various diseases. The bacterial isolates obtained from clinical samples were Escherichia coli, Salmonella sp., Staphylococcus aureus and Bacillus subtilis. After identification and confirmation the pure culture of clinical bacterial isolates were used to test the sensitivity of bacterial isolates against sample products. Dimethyl sulfoxide and distilled water were used as solvent for sample products. Different types of honey, sandal oil and black pepper were tested against bacterial isolates. Results of all sample products showed zone of inhibition against all selected bacterial isolates. At the dilution of 25µl of stock solution in 75µl and at 50µl: 50µl concentration, the results were similar as obtained from honey and sandal oil. Dimethyl sulfoaxide DMSO and honey 1 showed inhibition zones against all except Staphylococcus aureus, whereas honey 2 was effective against all isolates. Sandal 1 oil showed inhibition zones against all isolates except E.coli whereas sandal 2 was effective against all. The results obtained from black pepper 1 and 2 did not gave effective results both at 50µ litre: 50µ litre and 25µ litre: 75µ litre concentration, both were found to be effective against Bacillus subtilus. Standard medicine showed inhibition zone against all selected bacterial isolates in each test.

Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan