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A MICROPROPAGATION SYSTEM FOR CLONING OF HEMP (CANNABIS SATIVA L.) BY SHOOT TIP CULTURE

REN WANG^1*, LI-SI HE¹, BING XIA¹, JIN-FENG TONG¹, NING LI² AND FENG PENG¹

Abstract: This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·L^-1sucrose, 5.5 g·L^-1agar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L^-1TDZ, 0.1 mg·L^-1NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L^-1IBA and 0.05 mg·L^-1NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.

¹Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, (Mem. Sun Yat-Sen); Jiangsu province key laboratory for plant Ex-situ conservation, Nanjing, 210014, P.R. China

²Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong SAR, P.R. China