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WATER STRESS INDUCED VARIATIONS IN PROTEIN PROFILES OF GERMINATING COTYLEDONS FROM SEEDLINGS OF CHICKPEA GENOTYPES

N. BIBI^*, A. HAMEED, H. ALI, N. IQBAL, M. A. HAQ, B.M. ATTA, T.M. SHAH AND S.S. ALAM

Abstract: Studies were planned to analyze the response of chickpea genotypes to water stress (10% - PEG) at germination stage. Four genotypes viz., CM-2000, CM-94/99, CM-98 and 6153 were used and data for root length and protein mobilization from cotyledons to seedlings were collected on fifth and seventh day after germination. Analysis of variance revealed highly significant differences among varieties under control and stressed conditions. CM-94/99 showed significant increase (p<0.05) in root length while CM-2000 showed significant decrease (p<0.05) under the applied stress. SDS-PAGE was utilized to detect variations in protein profiles of these genotypes from 1^st till 7^th day. Quantitative decrease in some high molecular weight proteins was observed at 5^th day while some new germination related proteins appeared on 7^th day in controlled samples of all genotypes. However genotype specific variations were observed under the applied stress. In CM-2000 delayed expression (on 7^th day) of 100 kDa and 60.8 kDa proteins was observed under water stress while these proteins were expressed earlier (on 5^th day) in CM-94/99. Moreover expression of 39.6 kDa and 42 kDa proteins was also delayed (7^th day) in CM-2000 but no change in the expression of these proteins was observed in other genotypes.

Nuclear Institute of Agriculture and Biology, NIAB, Jhang road Faisalabad, Pakistan