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A facile procedure for efficient plantlet regeneration and fruiting without vernalization in Thellungiella salsuginea

Weiyang Sun^1,2, Jianfang Li², YANXIU ZHAO¹ AND Hui zhang¹*

Abstract: A facile and rapid system for efficient shoot regeneration and callus keeping was developed for Thellungiella salsuginea (Pall.) O. E. Schulz. Three kinds of explants including basal leaves, stems and anthers of vernalized plants were adopted here. Calli were formed from all these explants on MS medium supplemented with various plant hormone treatments. The highest callus inducing ratio for anther explants were observed on MS medium supplemented with 2.0 mg/l 2,4-D and 1.0 mg/l ZT zeatin, while for both stem and leaf explants 1.0 mg/l 2,4-D, 1.0 mg/l IBA and 0.1 mg/l ZT were used. The highest shoot regeneration ratios of anther and leaf calli were observed on MS medium with 0.1 mg/l IBA and 2.0 mg/l ZT and for stem calli it was 0.1 mg/l IBA and 1.0 mg/l ZT. Anther calli could keep vigorous growth on MS medium with 1.0 mg/l 2,4-D and 0.1 mg/l ZT. Even after three times subculture, shoots regenerated from anther calli could also develop flowers without vernalization while shoots regenerated from stem and leaf calli couldn’t. Without vernalization almost 100% of seedling regenerated from anther blossomed whereas only 7.3% and 3.0% of seedling from stem and leaf did respectively, which provided us not only a facile, rapid regeneration system but also an activator for research work of vernalization mechanism.

¹Biology Research Center of Shandong Normal University, Jinan 250014, P. R. China.

²College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250014, P. R. China.