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DEVELOPMENT OF GENETIC LINKAGE MAP OF LEAF HAIRINESS IN GOSSYPIUM HIRSUTUM (COTTON) USING MOLECULAR MARKERS

IFTIKHAR ALI^1*, ABIDA KAUSAR¹, MEHBOOB-UR-REHMAN³, YUSUF ZAFAR³, MUHAMMAD ASIF³, MUHAMMAD ASHRAF², SANA RIAZ¹, SARA ZAFAR¹, ABDUL WAHID², SOBIA MAQSOOD¹, MUBASHIR NIAZ¹ AND SYED QAISER ABBAS¹

Abstract: Cotton (Gossypium hirsutum L.) genotype Rajhans (pilose) was crossed with FH-634 (glabrous) and developed F₂ and F₃ segregating populations. PCR based techniques RAPD and SSR were used to determine DNA markers linked with the trait of hairiness using 400 RAPD and 54 SSR primers. Prior to analysis, PCR conditions were optimized. Eight RAPD primer markers OPD-19₆₄₀, OPF-11₆₃₀, OPG-06₉₈₀, OPG-17₅₀₀, OPH-13₁₁₀₀, OPN-14₈₉₀, OPO-11₉₂₀, OPO-14₁₂₀₀ were consistently detected in duplicate reactions. One SSR primer pair, JESPR-154 amplified a 150 bp DNA fragment in hairiness population. Polymorphic DNA markers were used to develop linkage map. Construction of genetic linkage map using RAPD and SSR markers revealed that the inheritance for leaf hairiness exists, which can be used to improve insect resistance in cotton.

¹Department of Botany, GC University, Faisalabad, Pakistan

²Department of Botany, University of Agriculture, Faisalabad, Pakistan

³National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan.

^*E-mail: iftikhar_pbd@yahoo.com