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CALLUS INDUCTION AND REGENERATION IN ELITE SUGARCANE CULTIVAR HSF-240

HAMID RASHID², SABAZ ALI KHAN¹, MUHAMMAD ZIA^1*, M. FAYYAZ CHAUDHARY¹, Z. HANIF³ AND ZUBEDA CHAUDARY⁴  

Abstract: Studies were carried out to establish an efficient system for callus induction and regeneration of sugarcane cultivar HSF-240. Shoot tip with 5-10 mm size was taken as explant for callus induction on MS medium containing different concentrations of 2, 4-D. Among the different concentrations of 2, 4-D used, maximum (80-82%) calli production with 3-4 mm in size were observed on media containing 2, 4-D, for both 2 mg/l and 3 mg/l. Optimum shoots length (8 mm) was obtained on MS medium containing 1.0 mg/l GA₃, 0.5 mg/l Kin and maximum roots (3.6) with maximum length of (3.5 mm) was obtained at 1.0 mg/l IBA.

¹Faculty of Biological Science, Quaid-i-Azam University, Islamabad, Pakistan

²Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan

³Institute of Biotechnology and Genetic Engineering (IBGE), NWFP Agricultural University, Peshawar, Pakistan,

⁴Department of Botany, Hazara University, Mansehra, Pakistan.