Pak. J. Bot., 41(4): 1651-1658, 2009. | Back to Contents | ||||
|
Updated: 03-09-09 | ||||
|
FFECT OF CYTOKININS ON SHOOT MULTIPLICATION IN THREE ELITE SUGARCANE VARIETIES
SABAZ ALI KHAN1, HAMID RASHID2*, M. FAYYAZ CHAUDHARY1, ZUBEDA CHAUDHRY3, ZARRIN FATIMA1, SADAR UDDIN SIDDIQUI4 AND MUHAMMAD ZIA1
Abstract: Studies were carried out for rapid micropropagation of three elite sugarcane varieties i.e., HSF-240, CP-77-400 and CPF-237. The explants were surface sterilized with 50% clorox for 30 minutes. The cultures were initiated by inoculating them on MS (Murashige & Skoog, 1962) medium containing 1.0 mg/l Kin along with 0.1 mg/l GA3. Multiplication of the cultures was obtained by using BAP and Kin in various combinations and concentrations in MS medium. The optimum multiplication for variety HSF-240 was obtained at 1.5 mg/l BAP, 0.5 mg/l Kin with 16.5 cm shoot length, 11 number of tillers and 32 number of leaves per plant. Similarly optimum multiplication for variety CP-77-400 was obtained at 1.0 mg/l BAP and 0.5 mg/l Kin, with a maximum of 8.5 cm shoot length, 7 number of tillers and 24 number of leaves. Best multiplication rate for variety CPF-237 was observed at 1.0 mg/l BAP and 0.1 mg/l Kin with a maximum of 12 cm shoot length, 6 number of tillers and 18 leaves per plant. Rooting of the plantlets was obtained on half strength MS medium containing 6% sucrose and various concentrations of IBA.
1Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 2Department of Bioinformatics, Mohammad Ali Jinnah University Islamabad, Pakistan 3Department of Botany, Hazara University, Mansehra, Pakistan 4Plant Genetic Resources, (IABGR), NARC Islamabad, Pakistan. |
||||
|
|||||
Back to Contents |
|
Back to Contents | |||
|