Pak. J. Bot., 41(4): 1759-1767, 2009. | Back to Contents | ||||
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Updated: 03-09-09 | ||||
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GENETIC DIVERSITY OF INDIGENOUS FENNEL (FOENICULUM VULGARE MILL.) GERMPLASM IN PAKISTAN ASSESSED BY RAPD MARKERS
N.Y. ZAHID1, N.A. ABBASI1*, I.A. HAFIZ1 AND Z. AHMAD2
Abstract: Fennel (Foeniculum vulgare Mill.) is an important, well-known aromatic and medicinal herb. Fifty accessions of fennel were collected from different parts of Pakistan and evaluated for important characteristics like seed germination percentage (ger %), days to initiation of flowering, plant height, stem girth, nodal distance, umbel diameter, days to 50% maturity, days to harvesting, seed yield per row, weight of 100 seeds, Harvest index (%). Genomic DNA of the accessions was extracted and subjected to RAPD analysis in order to ascertain their genetic diversity. Twenty-four out of 30 decimer primers generated 145 clear bands and 70 (48%) were polymorphic. Sixteen primers OPA-01, OPA-03, OPA-04, OPA-05, OPA-07, OPA-10, OPA-11, OPA-14, OPA-15, OPA-18, AC-11, AC-14, AC-15, AC-16, AC-18 and AC-20 gave polymorphism for different characters. About 66.6% of polymorphic primers generated the highest index to resolve genetic diversity even in small number of accessions. Seven accessions from Punjab, 3 from NWFP, one from Balochistan and one from Northern Areas of Pakistan had appeared with promising characters.
1Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi. Pakistan 2National Agriculture Research Centre, Islamabad, Pakistan. *Corresponding author E-mail: nadeemabbasi65@yahoo.com |
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