| Pak. J. Bot., 41(4): 1931-1939, 2009. | Back to Contents | ||||
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Updated: 03-09-09 | ||||
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VIRUS INDEXATION OF IN VITRO REGENERATED SUGARCANE PLANTS
SHAGUFTA NAZ1*, FAYYAZ AHMAD SIDDIQUI2, AAMIR ALI3 AND JAVED IQBAL4
Abstract: The present study reports the SCMV
indexation of In vitro regenerated plants by infectivity assay,
serology test and by transmission electron microscopy. The plants were
developed by apical meristem, organogenesis and embryogenesis methods.
For infectivity test sap of In vitro regenerated plants was
inoculated on different cultivars of Sorghum. Out of 166 plants only 44
were SCMV+tive. These plants were further analyzed by using precipitin
test. These plants also showed negative result for virus. A total of 17
randomly selected plants i.e., 2 from control, 5 each from apical
meristem, organogenesis and somatic embryogenesis were processed for
electron microscopy. The control plants showed virus particles. No virus
particles were detected from plants derived from apical meristem. Two
plants obtained from organogenesis and three from embryogenesis were
identified with virus particles. All the SCMV indexed plants were grown
in green house and monitored for mosaic symptoms at weekly intervals. 1Department of Botany, Lahore College for Women University, Lahore, Pakistan 2AgriBiotech, 14-Km Multan Road, Lahore, Pakistan 3Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan 4School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan. |
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