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GENETIC TRANSFORMATION OF Bar GENE AND ITS INHERITANCE AND SEGREGATION BEHAVIOR IN THE RESULTANT TRANSGENIC COTTON GERMPLASM (BR001)

M.K. DAUD^{1, 2}, M.T. VARIATH¹, SHAFAQAT ALI¹, MUHAMMAD JAMIL², MUHAMMAD TARIQ KHAN⁴, MOHAMMAD SHAFI^{1, 3} AND ZHU SHUIJIN ¹*

Abstract: The Agrobacterium-mediated genetic transformation technique in cotton using two genotype independent approaches were exploited. The segregation and inheritance of the Bar gene as well as various agronomic and fiber quality traits of the resultant transgenic cotton germplasm (BR001) and its background germplasm (Coker 312) were also studied. PCR and Southern blot analyses showed successful integration of the foreign gene in case of both approaches. Although the transformation efficiency of shoot apex culture method was higher than Pollen Tube Pathway via ovarian injection method, the number of plants developed in the soils was almost the same. Moreover, the Mendelian inheritance and segregation studies confirmed that a single nuclear dominant gene governed the herbicide resistance characters in BR001. The present study suggests that both genotype independent approaches can be utilized in order to save time as well as to avoid chimeras.

¹Institute of Crop Science, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, P.R. China.

²Department of Biotechnology & Genetic Engineering, Kohat University of Science & Technology (KUST) 26000, Pakistan

³Department of Agronomy, NWFP Agricultural University, Peshawar, Pakistan

⁴National-IPM Program, National Agricultural Research Center (NARC), Islamabad 45500, Pakistan