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GENETIC DIVERSITY IN ANNUAL WILD SOYBEAN (GLYCINE SOJA SIEB. ET ZUCC.) AND CULTIVATED SOYBEAN (G. MAX. MERR.) FROM DIFFERENT LATITUDES IN CHINA

WEI AN¹, HONGKUN ZHAO², YINGSHANDONG^1,2*, YUMIN WANG², QIYUN LI² , BINGCHANG ZHUANG², LEI GONG¹ AND BAO LIU¹*

Abstract: A total of 50 accessions of annual wild soybean (Glycine soja) and cultivated soybean (G. max) were used to study genetic variations by three genetic fingerprinting systems, RAPD, SSR, and AFLP. Ninety polymorphic RAPD bands were generated by 10 decamer primers, 56 SSR loci were obtained by 12 pairs of primers and 935 bands were resolved by 11 AFLP primer combinations. Based on the marker data, an UPGMA-cluster analysis separated the soybean collections into two groups that corresponded to wild soybean and cultivated soybean respectively. Genetic diversity calculation suggested that the diversity of G. soja was higher than that of G. max. Specific informative bands for G. soja and G. max were detected, which provided molecular evidence in support of the clear distinction of G. soja and G. max. Finally, it was found that environmental factors may play important roles in soybean evolution. A comparison of the three molecular markers suggests that AFLP and SSR are more suitable than RAPD for genetic diversity studies in soybean.

¹Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China, ²Jilin Academy of Agricultural Science, Changchun 130124, China. Corresponding author: ysdong@cjaas.com or baoliu@nenu.edu.cn