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AN EFFICIENT PROTOCOL FOR RAPID MULTIPLICATION OF BRYOPHYLLUM PINNATUM AND BRYOPHYLLUM DAIGREMONTIANUM

SHAGUFTA NAZ^*, SUMERA JAVAD^*, SAIQA ILYAS^* AND AAMIR ALI^**

Abstract: A rapid multiplication protocol for two species of Bryophyllum pinnatum and Bryophyllum daigremontianum has been optimized in the present study.  Leaf sections of 1x1cm² were used as explants. The effect of various concentrations of thidiazuron TDZ (2.5, 5.0, 10.0, 15.0, 20.0 µM) and BAP alone and their combinations on MS medium were examined. TDZ proved more potent as its lower concentrations produced multiple shoots in B. daigremontianum i.e. 13.4^b±1.22 in 5 µM TDZ and 20.2^a±0.33 in 10 µM TDZ along with roots. The regeneration frequency and number of shoots per explant were also enhanced on these concentrations. Supplementation of media with different concentrations of BAP (1, 2, & 3 µM) and combinations of BAP and NAA did not improve shoot regeneration. Only shoots were produced from leaf sections in lower concentrations of BAP (1 µM) while the higher concentration of BAP did not show the optimum response. These micro shoots were then transferred to selected combinations of TDZ for further multiplication. TDZ 10 µM gave better results for shoot proliferation and elongation in both varieties. Plantlets produced by this method were shifted in green house for acclimatization. The best survival rate was  91% in 75% sand with 25% coco peat for B.pinnatum and 94% was noticed in 33% sand with 33% farmyard and leaf mould in B. daigremontianum.

^*Department of Botany, Lahore College for Women University, Lahore, Pakistan

^**Department of biological Sciences, University of Sargodha, Sargodha, Pakistan