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Pak. J. Bot., 41(6): 2783-2789, 2009.

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GENETIC ANALYSIS FOR DISTORTED SEGREGATION OF EF-1 GENE IN RICE

MUHAMMAD JAMIL¹, KUI JAE LEE² AND EUI SHIK RHA^3*

Abstract: The present study was carried out to assess the genetic mechanism of the distorted segregation of earliness-1 gene (Ef-1) in cross between Indica and Japonica linkage tester. The linkage relationship was investigated in F₂ populations between the Ef-1 gene on chromosome 10 and a new gametophyte gene. Distorted segregation of Ef-1 appeared in F₂ populations and it was not related to the F₁ seed sterility and the F₂ seed fertility. The ratios of the distorted segregation did not vary significantly among F₂ populations derived from F₁ hybrids of the same cross combinations. Ef-1 gene in B₁F₁ hybrids derived from the reciprocal backcrosses clearly showed that the distorted segregation was caused by a new gametophyte gene. The recombination value was calculated to be 24.2 ± 5.4% between Ef-1 gene and gametophyte gene.

¹Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology (KUST), Kohat 26000, NWFP, Pakistan

²College of Environmental & Bioresource Sciences, Chonbuk National University, Jeonju 570-752, Korea

^3*College of Bio industry Science, Sunchon National University, Suncheon 540-742, Korea