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Pak. J. Bot., 41(6): 2801-2806, 2009.

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EFFECT OF AGE OF EMBRYOGENIC CALLUS ON PLANT REGENERATION IN LOCAL CULTIVARS OF WHEAT (TRITICUM AESTIVUM L.)

NAVEED IQBAL RAJA, ASGHARI BANO, HAMID RASHID^1*, M. HAROON KHAN¹ AND ZUBEDA CHAUDHRY²

Abstract: High frequency regeneration system is a prerequisite for production of transgenic plants. The present study was conducted to study the age of callus on high frequency regeneration protocol for seven different wheat (Triticum aestivum L.) cultivars viz. Chakwal-97, Inqualab-91, Punjnand-2000, Manthar-2002, Kohsar-95, Margalla-99 and C-591. Mature embryos of these cultivars were inoculated on MS (Murashige and Skoog, 1962) and N₆ (Chu, 1978) media supplemented with 2.5 mg/l 2, 4-D. Callus induction started after two weeks. These calli were placed on regeneration medium supplemented with BAP at 5 mg/l and IAA at 1mg/l) after 14, 18, 22, 26, 30, 34, 38, 42, 44, 48, 52, 56 and 60 days. Regeneration frequency was noted after 6-8 weeks of inoculation on regeneration medium. It was found that regeneration frequency was highest i.e., 95% by Chakwal-97 in 26 days old calli, whereas lowest at 48 days i.e., 13%. From these studies it was concluded that 3-4 weeks old mature embryo derived calli were more effective for highest regeneration frequency.

Department of Plant Sciences, Quaid-i-Azam, University Islamabad, Pakistan

¹Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan,

²Department of Botany, Hazara University, Mansehra, Pakistan.