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IN VITRO  PLANT REGENERATION IN BREAD WHEAT (TRITICUM AESTIVUM L.)

SHAFQUAT YASMIN, IMTIAZ AHMED KHAN, ABDULLAH KHATRI, NIGHAT SEEMA, GHULAM SHAH NIZAMANI AND MOHAMMAD AFZAL ARAIN

Abstract: Callus was induced on immature embryos of wheat (Triticum aestivum L.) cvs. Sarsabz, Khirman and Soghat-90. The application of different phytohormone concentrations yielded differential behaviour of genotypes for callus induction, mainly due to the genotype and media interaction. Variety Khirman produced highest callus on media (M1). Callus proliferation was also influenced by genotype and media interaction. Best callus proliferation was observed in Khirman on media (M4). Data revealed that Soghat-90 showed weakest interaction with present media compositions used in this study. Similarly regeneration potential was also subjective to media and genotype interaction. Data on chlorophyll mutants were also collected to study the frequency of genetic variation produced by each variety due to media effect. Green plantlets were transplanted in the field after weaning for evaluation of genetic variability.

Nuclear Institute of Agriculture, Tando Jam, Sindh, Pakistan.