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Pak. J. Bot., 42(1): 155-163, 2010.

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TISSUE CULTURE STUDIES IN TOMATO (LYCOPERSICON ESCULENTUM) VAR. MONEYMAKER

ZUBEDA CHAUDHRY¹, SIDRA ABBAS², AZRA YASMIN², HAMID RASHID^3*, HABIB AHMED¹ AND MUHAMMAD AKBAR ANJUM⁴

Abstract: A protocol was developed for callus induction and regeneration in tomato (Lycopersicon esculentum) var. Moneymaker. Hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. Explants were cultured on MS medium having different concentrations of hormones. Maximum callogenesis from hypocotyls was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 mg/l), BAP (5 mg/l) and Kin (4 mg/l). It was 65.2% for hypocotyls. For leaf discs maximum callogenesis was achieved on MS medium supplemented with IAA (2 mg/l), NAA (2 mg/l), BAP (2 mg/l) and Kin (4 mg/l). It was 81.3% for leaf discs. Calli were cultured on MS medium having concentrations of Zeatin (1 mg/l) and IAA (1 mg/l) for regeneration. They showed maximum regeneration of 69.2% from hypocotyls. Minimum regeneration of 2.8% was obtained on MS medium supplemented with IAA (2 mg/l), NAA (2 mg/l), BAP (2 mg/l) and Kin (4 mg/l). Tomato shoots were shifted to ½ MS medium containing IBA (0.1 mg/l) and BAP (0.0025 mg/l) for rooting and all responded positively to rooting.

¹Department of Botany, Hazara University, Mansehra, NWFP, Pakistan.

²Environmental Sciences Programme, Fatima Jinnah Women University, The Mall, Rawalpindi,

³Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan.

⁴University College of Agriculture, Bahauddin Zakariya University, Multan