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PRODUCTION OF ALPHA AMYLASE FROM A RANDOMLY INDUCED MUTANT STRAIN OF BACILLUS AMYLOLIQUEFACIENS AND ITS APPLICATION AS A DESIZER IN TEXTILE INDUSTRY

I. HAQ*, S. ALI, M.M. JAVED, U. HAMEED, A. SALEEM, F. ADNAN AND M.A. QADEER^†

Abstract: The present study is concerned with the improvement of Bacillus amyloliquefaciens strain UNG-16 for alpha amylase production. The bacterial culture was exposed to UV irradiation at 1.6×10² J/m²/S for 15-60 min. However, UV induced viables did not give improved alpha amylase production; therefore chemical mutation using ethyl methane sulphonate (EMS 50-300 µl/ml) was undertaken for 10-60 min. The mutant B. amyloliquefaciens EMS-6 gave 102.78±2.22 U/ml/min enzyme activity which was 1.4 fold higher than the parental strain. In stirred fermentor, the incubation period was reduced from 72 to 48 h after inoculation. The production of alpha amylase was found to be maximal when the 60% volume, 2.0 vvm air supply and 400 rpm agitation rate was maintained during the fermentation period. The incubation temperature (37ºC) and size of inoculum (8.0 %) were also optimized. A 100% desizing of grey fabric (or starch removal) was obtained with 200-250 enzyme units at pH 6.5 at 60ºC in 1 h.

Institute of Industrial Biotechnology (IIB), GC University Lahore, Pakistan

*Corresponding author E-mail: ikrhaq@yahoo.com; ^†Visiting Professor