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Pak. J. Bot., 42(1): 605-614, 2010. |
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Updated: 08-03-10 | ||||
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GENETIC VARIABILITY IN MUTATED POPULATION OF SUGARCANE CLONE NIA-98 THROUGH MOLECULAR MARKERS (RAPD AND TRAP)
IMTIAZ AHMED KHAN, SAJIDA BIBI, SHAFQUAT YASMIN, ABDULLAH KHATRI, NIGHAT SEEMA AND SHAHID AFGHAN*
Abstract: Variability obtained from mutation breeding (gamma rays) was examined through molecular marker techniques (RAPD). A total of 85 loci were amplified, out of which 76.47% were polymorphic and 23.53% were monomorphic. Fragments size ranged from 220bp-2.1kb and fragments produced by various primers ranged from 3-13 with an average of 5 fragments per primer. The highest number of loci (13) was amplified with primer B-07, while the lowest number 3 with primer B-01. Results revealed that mutant P1 (20Gy) contained a specific segment of 2.03kb. Genetically most similar genotypes were P2 (10Gy) and P4 (20Gy) (95.55%) while most dissimilar genotypes were P4 (10Gy) and P3 (20Gy) (63.2%). On the basis of results achieved, the mutants could be divided into four clusters and three groups. Mutants P4 (40 Gy) and P4 (10 Gy) were genetically distinct from other mutants.
Plant Breeding and Genetics Division, Nuclear Institute of Agriculture, Tando Jam, Pakistan *SSRI, Jhang |
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