| Pak. J. Bot., 42(2): 681-684, 2010. | Back to Contents | ||||
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Updated: 20-05-10 | ||||
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IN VITRO POLLEN GERMINATION CAPACITY OF CITRULLUS LANATUS L., (CUCURBITACEAE)
SHAUKAT ALI KHAN AND ANJUM PERVEEN
Abstract: Pollen germination capacity of
Citrullus lanatus L., (Cucurbitaceae) in hanging drop technique
was evaluated up to 48 weeks. The collected pollen were stored at
different temperatures (4ēC, -20ēC, -30C and -60ēC). The pollen were
also treated in organic solvents (acetone, benzene & chloroform), in
vacuum over silica gel and in freeze dryer (-60ēC) for 30 minutes. The
study indicates that low temperature is far better than high temperature
with respect to pollen germination capacity and viability. In organic
solvents benzene showed better results as compared to vacuum dried
pollen. Freeze dryer (-60ēC) seems to be the best method to store pollen
grains for a long period of time. Department of Botany, University of Karachi, Karachi-75270, Pakistan. |
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