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DETERMINATION OF TAXONOMIC STATUS OF CHINESE SPECIES OF  THE  GENUS  CLEMATIS  BY  USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY (HPLC-MS) TECHNIQUE

MUHAMMAD ISHTIAQ CH^{1, 2}*, Q. HE², S. FENG, YI WANG², P.G. XIAO², YIYU CHENG²* AND HABIB AHMED³

Abstract: A comparative taxonomic study using chemometric and numerical taxonomic approaches on 15 populations of 12 species of major taxa of the genus Clematis, belonging to sections, Rectae, Clematis, Meclatis, Tubulosae and Viorna were analyzed by HPLC coupled with diode array detector and ESI-MS. The chemodiversity profile of saponins proved to be useful taxonomic markers for the genus and results are presented in phenograms. The compound ‘Huzhangoside D’ was the most abundant in analyzed species of the genus. Numerical taxonomic study was also conducted based on phylogenetically informative characters which corroborated with chemical fingerprinting findings. The significance of chemical markers in taxonomic study as well as their correlation between morphology and chemical compound profile is also debated with its significant role in botanic drugs identification.

¹Department of Botany, Mirpur University of Science & Technology (MUST) Bhimber Campus, (AK) Pakistan

²Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Institute of Bioinformatics, Zhejiang University, No. 38 Zhe da Road Hangzhou, 310027, P. R. China

³Department of Botany, University of Hazara, Garden Campus Mansehra, NWFP, Pakistan.