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DNA LANDMARKS FOR GENETIC RELATEDNESS AND DIVERSITY ASSESSMENT IN PAKISTANI WHEAT GENOTYPES USING RAPD MARKERS

MUHAMMAD FAISAL SIDDIQUI^1*, SAEED IQBAL¹, SHAZIA ERUM², NAHEED NAZ¹, SAJID KHAN¹

Abstract: DNA profiles from 10 Pakistani wheat genotypes were evaluated for diversity assessment based on RAPD markers. A total of 79 DNA fragments were generated by 10 RAPD primers, with an average of 7.9 bands primer^-1. Of these, 64 fragments (81%) were polymorphic among 10 genotypes. Genetic diversity was evaluated via UPGMA cluster analysis by constructing dendrogram, which were used for the calculation of similarity coefficients between these genotypes. The greatest similarity (95%) was observed between PR-94 and PR-95, whereas PR-96 with PR-90 showed the lowest similarity (60%). Adoption of this technology would be useful to the plant protection regulatory systems, especially for plant variety identification and registration of new plant varieties, breeding programs and protection purposes.

¹Federal Seed Certification and Registration Department (FSC & RD), Islamabad, Pakistan

²Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre (NARC), Islamabad, Pakistan

*Corresponding author: Email- address: send2biotech@yahoo.com; Tel: +92-3339017644