Pak. J. Bot., 42(2): 1093-1102, 2010. | Back to Contents | ||||
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Updated: 20-05-10 | ||||
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COMBINED EFFECT OF CYTOKININ AND SUCROSE ON IN VITRO TUBERIZATION PARAMETERS OF TWO CULTIVARS i.e., DIAMANT AND RED NORLAND OF POTATO (SOLANUM TUBEROSUM)
*AAFIA ASLAM AND JAVED IQBAL
Abstract: The present study is focused on the
optimization of culture media and explant density of apical and nodal
segments of two potato cultivars for In vitro microtuber
production. Fifty media manipulations of Murashige & Skoog (1962) were
tested by varying concentrations of sucrose (4, 6, 8, 10 and 12%) either
alone on in combination (5-8% sucrose) with kinetin and BA. Different
parameters with regard to microtuber induction mean number of
microtubers, mean fresh weight of microtubers both from single and
multinode explant of Diamant and Red Norland were studied. Medium MB8
(5mg BA and 8% sucrose) in term of induction and mean number of
microtubers per single node explants and MB11 (6mg BA and 7% sucrose) in
terms of mean fresh weight per single node provided best results for cv.
Diamant. In case of Red Norland, medium MK10 (2mg Kinetin and 6%
sucrose) in term of induction, mean number and mean fresh weight of
microtubers per multinode explants gave best results. In comparison, cv.
Diamant was better for microtuber induction and development than cv. Red
Norland. Seed Centre, University of the Punjab, Quaid-e-Azam Campus Lahore, Pakistan 54590 |
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