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ANTIFUNGAL AND NEMATICIDAL ACTIVITY OF SELECTED LEGUMES OF PAKISTAN

SHAKEEL AHMAD¹, MUSSARAT AKHTER^2*, M. ZIA-Ul-Haq³, MEHJABEEN⁴ AND SAGHEER AHMED⁵

Abstract: The antifungal activity of legume seed extracts was tested against 6 fungi, viz., Trichophyton longifusus, Candida albicans, Aspergilus flavus, Microsporum canis, Fusarium solani and Candida glaberata. The extracts showed moderate activity against different fungal strains. Nematicidal activity has also been carried out to evaluate their potential toxicity against juveniles of the root-knot nematode Meloidogyne spp. In vitro results showed that ethanolic extract of these legumes caused appreciable mortality of second stage juveniles of Meloidogyne javanica and Meloidogyne incognita. The concentrations used @ 1% and 0.5% were found more effective and produced significant results as compared to 0.25%, and 0.1%. The mortality rate increased with increasing exposure time for most of the extracts.

¹Department of Agronomy, ³Department of Chemistry, Bahauddin Zakariya University, Multan-60800, Pakistan

²Food & Marine Resources Research Center, PCSIR Labs Complex, Karachi-75280, Pakistan

⁴Department of Pharmacology, Federal Urdu University of Arts, Science & Technology, Karachi-75300, Pakistan

⁵Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi-74800, Pakistan