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IN VITRO CONSERVATION OF CADABA HETEROTRICHA STOCKS, AN ENDANGERED SPECIES IN PAKISTAN

HAIDER ABBAS^1* AND MUHAMMAD QAISER²

Abstract: In vitro conservation protocol was developed for Cadaba heterotricha, an endangered species reported from Southern Pakistan. This species has been subjected to various threats including habitat loss and over-exploitation, need an urgent conservation measures. Seeds collected from the wild, were used as initiating material. While, nodal segments of in vitro germinated seedlings were employed to induce multiple shoot regeneration. Full strength MS medium supplemented with 2.5 mg/l BAP and 0.5 mg/l Kin along with 0.5 mg/l NAA helps in attaining the highest number (16.0) of shoots with 100% shoot regeneration frequency. The cultures maintained in good conditions with subculture after 6-7 months. IAA at the level of 3.0 mg/l gave maximum number (2.70) of rooting along with the highest rooting frequency (80%).

¹Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi-75270, Pakistan,

²Federal Urdu University of Arts, Science and Technology, Karachi -75300, Pakistan,

^*Corresponding author: invitro.life@gmail.com