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Pak. J. Bot., 42(4): 2257-2267, 2010. |
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Updated: 09-12-10 |
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IMPROVEMENT OF SELECTED STRAINS THROUGH GAMMA IRRADIATION FOR ENHANCED LIPOLYTIC POTENTIAL
TEHREEMA IFTIKHAR1*, MUBASHIR NIAZ1, YASIR HUSSAIN1, SYED QAISER ABBAS1, IFFAT ASHRAF2 AND MUHAMMAD ANJUM ZIA3
Abstract: The purpose of the present investigation was to enhance the production of industrially important enzyme lipase by subjecting the wild lipase producing fungal strains i.e. Aspergillus niger, Rhizopus microsporus and Penicillium atrovenetum to various doses of gamma irradiation (20, 40, 60, 80, 100, 120, 140 and 160 Gy). The isolation and lipolytic activity of selected mutant derived strains is described in this paper. Among all the mutants tested, MBL-5 obtained at 140Gy of Aspergillus niger strain showed highest extracellular lipase activity (13.75 ± 0.15 U mL-1) while MBL-1 Rhizopus microsporus @ 20Gy showed the lowest activity i.e., 1.06 ± 0.11 U mL-1. A range of pH 3, 5, 7, 9 and 11 was used to check the lipolytic potential of various mutants alongwith their wild type. It was observed that MBL-5 (Aspergillus niger) and MBL-2 (Rhizopus microsporus) showed enhanced extracellular lipase activity at pH 11 while MBL-3 (Penicillium atrovenetum) showed the highest extracellular lipase activity 22.53 ± 0.21 U mL-1 at pH 9. It indicates a possible role for the MBL-2, MBL-3 and MBL-5 mutant strains in the detergent industry for the development of eco-friendly technologies.
1Department of Botany, GC University, Faisalabad, Pakistan 2Department of Environmental Sciences, GC University, Faisalabad, Pakistan 3Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan *Corresponding author: pakaim2001@yahoo.com, 92-42-36854508 (Res), 92-41-9201488 (Off) |
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