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Pak. J. Bot., 42(4): 2895-2902, 2010. |
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Updated: 09-12-10 |
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EXPRESSION PATTERN OF TRICHODERMA CELLULASES UNDER DIFFERENT CARBON SOURCES
NIGHAT ASLAM1, MUNIR A SHIEKH1, MUHAMMAD ASHRAF2 AND AMER JAMIL1*
Abstract: Production of easily fermentable low-cost sugars demands
for an economic and less expensive method of enzymatic hydrolysis of
cellulosic biomass. It is highly desirable to compare various
fermentation media with different carbon sources for cellulase
production. Trichoderma harzianum was grown on different carbon
sources and monitored for cellulase production. Glucose-grown cultures
of T. harzianum showed high amount of mycelial growth but no
yield of cellulase enzyme. Cellulase expression was also studied herein
by comparing the cellulase activities using soluble and insoluble
cellulosic carbon sources in the growth media in order to obtain less
expensive fermentation media. Outcome of the research will be helpful in
the development of low cost system for production of cellulose. 1Molecular Biochemistry Lab., Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad- 38040. Pakistan. 2Department of Botany, University of Agriculture, Faisalabad. 38040. Pakistan. 2Second Affiliation: King Saud University, Riyadh, Saudi Arabia. |
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