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Pak. J. Bot., 42(4): 2903-2910, 2010. |
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Updated: 09-12-10 |
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EVALUATION OF BACILLUS
THURINGIENSIS ISOLATES AGAINST ROOT-KNOT NEMATODES FOLLOWING SEED
APPLICATION IN OKRA AND MUNGBEAN
Abstract: Bacillus thuringiensis, an insecticidal bacterium is widely used as a
biological control agent for a number of insects. During a survey, 10
isolates of Bacillus thuringiensis were isolated from rhizosphere
of okra, brinjal, tomato, cotton, cabbage, onion and water melon crops
cultivated in Karachi, Thatta, Hyderabad and Nawabshah. These isolates
were evaluated for their nematicidal potential under laboratory and
green house experiments. In laboratory studies, eggs and Juveniles were
exposed to different BT isolates at 50% concentration of cell free
filtrate for different time intervals. The cell free filtrate not only
inhibited the hatching of eggs but also killed the second stage
juveniles at different time intervals. In green house studies seed
treatment of okra (Abelmoschus esculentus
(L.) Moench var Arka anamika) and Mung bean (Vigna radiata
[L.] Wilczek cv.MN-95) with cell suspension of BT isolates improved
plant growth and reduced number of galls, Egg masses, Eggs/egg mass,
Nematode population /g roots and Nematode population /200g soil as
compared to non-treated control.
Department of Botany,
University of Karachi, Karachi-75270, Pakistan |
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