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Pak. J. Bot., 42(4): 2941-2947, 2010. |
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Updated: 09-12-10 |
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MOLECULAR AND EPIDEMIOLOGICAL ANALYSIS OF
MULTIDRUG-RESISTANT SALMONELLA PARATYPHI A OBTAINED FROM
DIFFERENT REGIONS OF PAKISTAN
Abstract: Incidence of paratyphoid infections caused by Salmonella
paratyphi A is significantly mounting high in the country.
Indiscriminate use of antibiotics which often results in the development
of drug-resistant strains, is making the treatment difficult. This
situation becomes worst when organism acquire multidrug resistance
(MDR). In the present study, various isolates of MDR S. paratyphi
A, obtained from different regions of Pakistan, were analyzed for
plasmid profile and multiplex PCR to determine the resistance gene
associated with plasmid and chromosomal DNA of S. paratyphi A. Multi-drug resistant isolates of S. paratyphi A
obtained during the year 2001-2006 from different regions of Pakistan
have been included in this study. These isolates showed resistance to
chloramphenical (30µg), ampicillin (10µg),
trimethoprim-sulfamethoxazole (23.5µg), and tetracycline (30µg).
Some strains
were also found resistant to ofloxacin and nalidixic acid. The plasmids
from bacterial strains were found to be approximately 220 kbp, harboring
antibiotic resistance genes. A multiplex PCR was used to identify any
variation in antibiotic resistance genes encoding the MDR phenotypes in
clinical isolates of S. paratyphi A. A homogenous pattern of
multiplex PCR product revealed that MDR isolates of S. paratyphi
A harbored the same resistance genes.
This
study will help taking effective measures for controlling disease in the
region, and the data could be used for future medical reference.
1Department of Microbiology, Federal Urdu University of
Arts, Science & Technology, Karachi |
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