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PROTEOMICS APPROACH TO IDENTIFY DIFFERENTIALLY EXPRESSED PROTEINS INDUCED BY IRON DEFICIENCY IN ROOTS OF MALUS

JING-YING WANG^{1, 2}, SONG-LIN RUAN³, WEI-HUA WU⁴, XUE-FENG XU¹, YI WANG¹ AND ZHEN-HAI HAN^1*

Abstract: Malus xiaojinensis (Malus xiaojinensis Cheng et. Jiang) is a Fe-efficient apple. We report for the first time a systematic proteomic approach to iron stress-responsive proteins in roots of Malus. Plantlets of Malus were cultured in hydroponic system without Fe element for 3 d. After then, total proteins were extracted from roots of the treated plantlets and separated by two-dimensional gel electrophoresis (2-DE). More than 700 protein spots were reproducibly detected. The matrix-assisted laser desorption/ionisation-time of flight and peptide mass fingerprinting (PMF) analysis and database searching helped us identify 12 up-regulated protein spots representing 10 different proteins. Two spots were putative pyridoxine biosynthesis protein isoform B, 2 were identified as s-adenosylmethionine synthetase (SAMS) and other 8 as fructokinase (FK), isocitrate dehydrogenase (NADP), monodehydroascorbate reductase (MADR), AP003246 NID, actin, Hypothetical protein, probable catechol O-methyltransferase (COMT) and vacuolar H⁺-ATPase (V-ATPase) catalytic subunit, respectively. These proteins were involved in regulation of carbohydrate and energy metabolism, stress-defense response, and membrane transport. Something relevant to Fe deficiency stress was discussed.

¹Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Trees

Institute for Horticultural Plants, China Agricultural University, Beijing, 100193, China;

²College of Plant Science, Jilin University, Changchun, 130062, China;

³Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou, 310024, China;

⁴State Key Laboratory of Plant Physiology and Biochemistry, College of Biological, China Agricultural University, National Plant Gene Research Center, Beijing, 100193, China.