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IN VITRO SCREENING OF METHANOL PLANT EXTRACTS FOR THEIR ANTIBACTERIAL ACTIVITY

TARIQ HUSSAIN, MUHAMMAD ARSHAD, SARZAMIN KHAN, HAMID SATTAR AND MUHAMMAD SUBHAN QURESHI

Abstract: The purpose of this study was to observe the antibacterial activity of aqueous methanolic extracts of 10 plants against 2-gram negative bacteria (Pasteurella multocida, Escherichia coli) and 3-gram positive bacteria (Bacillus cereus, Staphylococcus aureus, Corynebacterium bovis) by using disc diffusion method. The minimum inhibitory concentration (MIC) was determined by agar well diffusion method and agar dilution method. All the bacteria were susceptible to different plant extracts. Lawsonia inermis, Embellia ribes and Santalum album showed antibacterial activity against all the tested bacteria. The extract of Santalum album showed maximum antibacterial activity of the 10 plant extracts used. Bacillus cereus and Pasteurella multocida were the most sensitive bacteria against most of the plant extracts. It is clear from the results of the present studies that the plant extracts have great potential as antimicrobial compounds against bacteria. However, there is a need of further research to isolate the active ingredients for further pharmacological evaluation.

Faculty of Animal Husbandry and Veterinary Sciences, NWFP Agricultural University, Peshawar-25120, Pakistan

^*Department of Veterinary Microbiology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan.

^*Corresponding author: E-mail: dr.zaminaup@gmail.com; Tel. 0092-91-5702190, Mobile: 0092-300-8592606