| Pak. J. Bot., 43(1): 715-723, 2011. | Back to Contents | ||||
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Updated: 26-02-11 | ||||
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OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF ALPHA AMYLASE BY WILD AND MUTANT STRAIN OF ASPERGILLUS ORYZAE IN STIRRED FERMENTER
ROHEENA ABDULLAH*, IKRAM-UL-HAQ AND MOHSIN JAVID
Abstract: The present study deals the optimization of cultural conditions for the production of alpha amylase by Aspergillus oryzae IIB-30 and its mutant derivative A. oryzae EMS-18 in stirred fermenter. The time of fermentation for enzyme production by both wild and mutant strains was studied. It was found that the time required for maximal enzyme production (608 U/ml) in case of mutant strain was reduced to 48 h compared with 64 h by wild strain for maximum enzyme production (335 U/ml). The kinetic depiction of results showed optimal fermentation period for enzyme production to be 64 h and 48 h, respectively. The other cultural conditions such as initial pH (5), aeration rate 1.5 vvm (mutant), dissolved oxygen (15%), inoculum size (10%) and agitation intensity (200 rpm) were optimized for enzyme production.
Institute of Industrial Biotechnology (IIB), GC University Lahore, Pakistan *Corresponding author: roheena_abdullah@yahoo.com |
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