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Pak. J. Bot., 43(2): 1011-1020, 2011. |
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Updated: 07-04-11 | ||||
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EFFECT OF TEMPERATURE STRESS ON POLYPHENOL OXIDASE ACTIVITY IN GRAINS OF SOME WHEAT CULTIVARS
WAQAS KHAN KAYANI1*, SYED ABDUL MAJID2, TARIQ MAHMOOD3, SMS NAQVI4 AND ABDUL WAHEED5
Abstract: Color is a key quality trait of wheat-based products and polyphenol oxidase (PPO) is implicated to play a significant role in their undesirable darkening. Polyphenol oxidase catalyzes the oxidation of phenols to quinines, which auto oxidize and polymerize with amino acid of cellular proteins resulting brown and black pigmentation propounding reduced nutritional values. In present study, the PPO activity in 50 different Pakistani wheat cultivars was investigated and grouped into three categories viz; low, medium and high PPO activity cultivars. PPO is a heat labile enzyme. To investigate effect of heat stress, nine cultivars from each category were chosen for treatment at 30, 40 and 50°C for 30, 60, and 120 minutes each. A substantial change was experienced in PPO activity as compared to room temperature. Two wheat cultivar Wafaq-2001 and AS-2002 showed a compromising attitude of minimum PPO activity at 30°C for a period of 30 and 60 minutes of incubation. In general, an incubation of 30°C or 60°C (low or high) for a period of 30 minutes can be recommended for suppressing PPO activity.
1Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. 2Department of Botany, University of Azad Jammu and Kashmir, Muzaffarabad, Azad Kashmir, Pakistan. 3Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan 4Department of Biochemistry, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan 5COMSATS Institute of Information Technology, Sahiwal Campus, Sahiwal, Pakistan |
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