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INFLUENCE OF ELEVATED LEVEL OF CO₂ ON  BIOCHEMICAL PARAMETERS OF GLYCINE MAX (L.) MERR. INOCULATED WITH BRADYRHIZOBIUM JAPONICUM

NEELOFER HAMID, AFSHAN REHMAN AND FAIZA JAWAID

Abstract: Effect of elevated CO₂ with Bradyrhizobium japonicum strain (KUCC-118) on some biochemical parameters of soybean (Glycine max (L.) Merr was studied. The sterilized seeds of Glycine max were coated with Bradyrhizobium japonicum. The seedling of soybean was fumigated with 2% (20000ppm) and 3% (30000ppm) CO₂ for 10 and 20 mins., duration. Seedlings were exposed twice a day in a week to elevated CO₂. The 3% (3000ppm) CO₂ fumigated plants showed weekly reduction in carbohydrate, total protein, total DNA and total RNA as compared to the exposure of 2% (2000ppm) concentration of CO₂. Control plants which were grown in ambient level (330ppm) of CO₂ with inoculation of Bradyrhizobium japonicum showed significant increase in biochemical parameters.

Department of Botany, University of Karachi, Karachi-75270, Pakistan