| Back to Contents | Pak. J. Bot., 43(2): 1343-1349, 2011. | Back to Contents | |||
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Updated: 07-04-11 | ||||
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ECOLOGICAL SCREENING OF LIPOLYTIC CULTURES AND PROCESS OPTIMIZATION
FOR EXTRACELLULAR LIPASE PRODUCTION FROM FUNGAL HYPERPRODUCER
TEHREEMA IFTIKHAR1*, MUBASHIR NIAZ1,
MAMONA ANWER1, SYED QAISER ABBAS, MUHAMMAD SALEEM2 AND RUKHSANA
JABEEN3 Abstract: Present investigation describes the biosynthesis of extracellular lipases by various local fungal strains isolated from various lipid rich habitats of Faisalabad. The isolated cultures of Aspergillus niger, Penicillium chrysogenum, Rhizopus microsporus, Mucor mucedo, Alternaria alternata, Trichophyton sp., Fusarium semitectum, E (un-identified), Curvularia sp., Aspergillus flavus, G (un-identified), F (Mucor sp.) and H (Synnematous) were identified and screened for the extracelluler lipases production. Different environmental parameters such as pH, temperature, inoculum size, amount of substrate and incubation time were optimized for the selected hyper producer. It was found that maximum production of lipases by Trichophyton sp., was obtained after 48 h of batch fermentation. Similarly, the diluent pH of 7.0 and incubation temperature of 30°C were found optimum for enzyme production by the microorganism. The maximum production of lipases during the course of present studies was 65.20 ± 1.13a U/g.
1Laboratory
of Mycology & Biotechnology, Department of Botany,
Government College University Faisalabad, Pakistan *Corresponding author: pakaim2001@yahoo.com |
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