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PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF ERUCA SATIVA SEED

MUHAMMAD GULFRAZ¹, ALIA SADIQ¹, HIRA TARIQ¹, MUHAMMAD IMRAN¹, RAHMATULLAH QURESHI^2* AND ASYIA ZEENAT¹

Abstract: Antibacterial activity of various solvent extracts of Eruca sativa seed as well as seed oil was investigated against Gram+ve and Gram-ve bacterial strains. Maximum zone of inhibition was observed from seed oil followed by methanolic seed extracts from all bacterial strains compared with broad spectrum antibiotics gentamicine. MIC values of seed oil were within the ranges of 52-72 µg/ml as compared to 56-70 µg/ml standard antibiotic (Gentamicine). Proximate and Phytochemical analysis of seed of E. sativa showed presence of all essential phyto constituents required for promising traditional medicine. Analysis of seed oil by gas chromatography revealed that there was high concentration of Erucic acid (51.2%) followed by oleic acid (15.1%) and cis-11-eicosenoic acid (12.5%). In addition, minor quantities of other essential and non essential fatty acids were also present. Therefore the present study supports effectiveness of E. sativa seeds for its use in traditional medicine used in various human disorders.

¹Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Murree Road, Rawalpindi, Pakistan.

²Department   of Botany, Pir Mehr Ali Shah Arid Agriculture University, Murree Road, Rawalpindi, Pakistan.

^*Corresponding author: rahmatullahq@yahoo.com