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determınatıon
of GenetIc DIversIty In the VIcIa L.
(SectIon
VIcIa) By UsIng Sds-Page
İRFAN EMRE1*
Abstract:
The interspecific and intraspecific variations in some taxa of Vicia
is explained by SDS-PAGE method. In this study, nine Vicia L.
Taxa, collected from different regions of Turkey have been
studied for the analysis of seed storage protein profiles to examine
their relationship. The differences among species were observed and all
nine taxa were clearly identifiable from the protein patterns.
Electrophoretic data were documented by using a gel documentation system
(Bio-Rad, USA) and analysed by using Quantity 1-D analysis software and
a dendogram has constructed with 4.0 % tolerance in UPGAMA (Unweighed
Pair-Group Arithmetic Mean). The dendogram from SDS-PAGE analysis showed
that all studied taxa constituted two clusters. The first one consisted
of V. noeana var. noeana, V. noeana var. megalodonta,
V. truncatula, V. peregrina, V. michauxii and V. grandiflora
second one by V. mollis, V. hybrida, and V. assyriaca.
Present results showed that all studied species have similar total
protein content. But V. truncatula (86.837 μg/ml) and V.
hybrida (83.209 μg/ml) have highest total protein content.
Whereas V. grandiflora (65.860 μg/ml) has low total protein
content.
1Firat
University, Faculty of Education, Department of Primary Education,
Elazig, Turkey;
*E-mail:
irfanemre@gmail.com
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