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DIVERSITY
IN MAJOR SEED STORAGE
PROTEINS OF RICE LANDRACES OF PAKISTAN
ZAHIDA HASSAN PERVAIZ1*, SADIA TEHRIM1, M. ASHIQ RABBANI2,
M.S. MASOOD2 AND SALMAN A. MALIK1
Abstract:
Grain proteins from 173 rice landraces (Oryza sativa L.) were
electrophoretically separated by sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE). Densitometric scanning of the
electrophoretic profiles permitted the estimation of the relative
concentration of 25 polypeptide fractions, which were used as
variables for the calculation of similarity coefficients between these
accessions. A considerable variation was observed in glutelin subunits
and Wx gene product. Very low variation was observed in prolamin
subunits. Lower genetic distance based on total seed protein profile
may be attributed to narrow genetic base. This electrophoretically
detectable protein polymorphism in rice grain can be used in further
breeding proposes and variety development. Our results suggest that
screening of landraces on the basis of variation in seed storage
protein profile using SDS-PAGE could be highly effective tool to
identify valuable rice genetic resources.
1Department
of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam
University, Islamabad, Pakistan
2Institiute
of Agri-Biotechnology & Genetic Resources, National Agricultural
Research Center, Islamabad, Pakistan
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