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BREEDING AND
CHARACTERIZATION OF HOMOKARYOTIC
HETEROPLASMIC MALE STERILE LINES
IN RICE (ORYZA SATIVA)
QIMING MEI1,2
RONG XIE3, CHENGYUAN LIU3, XICHANG ZHOU2
AND YINGGUO ZHU1
Abstract:
Twelve different
Cytoplasmic Male-Sterile (CMS) lines were crossed with 18 rice
varieties. From the hybrid with japonica rice Nongken 58, twelve
homokaryotic-heteroplasmic male sterile lines were developed in B7F1
after successive backcrossing and selection for stable male sterility
and desirable agronomic traits such as flowering habit and high
outcrossing rate. The experimental results demonstrated that expression
of the CMS factors were influenced by the corresponding nuclear genes.
Three pollen abortion types, including the typical, the spherical and
the stained abortion, were observed in the homokaryotic-heteroplasmic
male sterile lines. Formation of the aborted pollen grains was
influenced by the interaction among specific cytoplasmic and the
corresponding nuclear genes. As the CMS carriers, these
homokaryotic-heteroplasmic lines will have significant impact on the
utilization of multiple types of CMS in hybrid rice breeding. What is
more important is that these CMS lines are the invaluable materials for
the investigation of the molecular mechanism of CMS formation in
rice.
1The
College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
2The
Center of Gardens , Wuhan University, Wuhan 430072, P. R. China.
3Rice
and Sorghum Research Institute, Sichuan Academy of Agricultural Science,
Luzhou 646000 P. R. China
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