Pak. J. Bot., 43(3): 1733-1734, 2011.

Back to Contents	Pak. J. Bot., 43(3): 1733-1734, 2011.	FREE FULL TEXT PDF	Back to Contents
			Updated: 18-06-11
	*NOVEL PROTOCOL FOR ALBUMIN AND GLOBULIN DETECTION IN PISUM SATIVUM* GENOTYPES USING SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)** M. NISAR¹, A. GHAFOOR², M.R. KHAN³, SADDRUDINE SIDDIQUI², NASRULLAH KHAN⁴ AND MUHAMMAD FAHEEM SIDDIQUI⁴ Abstract:* In the present study a novel protocol for the removal of Albumin and Globulin in 10 genotypes of Pisum sativum were developed by using Osborn solubility class protein extraction buffer (10mM Tris-Hcl buffer, pH 6.8, 0.5 M NaCl). One major allocation of protein band and one minor protein band corresponding to 10 kda and 22 kda, respectively, was detected in SDS-PAGE (untreated cases). There is a need to further improve this method so that the minor groups of albumin and globulin could also be investigated further to characterize through 2D gel electrophoresis. ¹Department of Botany, University of Malakand, Chakdara, Dir (Lower), Khyber Pakhtoonkhwa, Pakistan ²Institute of Agri-biotechnology and Genetic Resources, NARC, Islamabad, Pakistan ³Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Pakistan ⁴Department of Botany, Federal Urdu University, Karachi, Pakistan *Correspondence author E-mail: mnshaalpk@yahoo.com; nisarhu@gmail.com

Back to Contents			Back to Contents
	Your browser does not support inline frames or is currently configured not to display inline frames.

*Correspondence author E-mail: mnshaalpk@yahoo.com; nisarhu@gmail.com