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EVALUATION OF PAKISTANI WHEAT GERMPLASM FOR BREAD QUALITY
BASED ON ALLELIC VARIATION IN HMW GLUTENIN SUBUNITS
AQSA TABASUM*, NAYYER IQBAL, AMJAD HAMEED AND RUBINA ARSHAD
Abstract:
Seventy
six Pakistani wheat genotypes including land races were investigated for
Bread quality
(BQ) based on allelic variation
in HMW glutenin subunits
at the Glu-1 loci through SDS-
polyacrylamide gel electropherosis. Twenty five different allelic
combinations were detected with a total of 14
Glu-1 loci.
Highest
polymorphism was revealed by Glu-B locus and some single/ rare sub units
were also screened out. The
frequencies
of dominant subunits were 50% for 2*,
42.11% for subunit pair
17+18 and
48.68% for 5+10
and 2+12 respectively.
The quality
scores displayed a range from 4 to 10,
however
generally good quality score of eight was more frequent (39. 47%). The
highest quality scores of 10 and 9 were observed in 22.36% and 19.74% of
genotypes respectively. The UPGMA analysis grouped genotypes into three
major with two additional sub clusters for each. The cluster “a” “b” and
“C” were separated at 73% genetic distance which was further
differentiated at a genetic distance of 50% into their sub clusters.
Pakistani wheat varieties/land races exhibited large variation in term
of HMW-GS. The generated information
will lead to the
pyrimiding
of sub units for high BQ through mission oriented marker assisted
breeding programes for quality improvement of wheat.
* Nuclear
Institute for Agriculture & Biology, Jhang Road, Faisalabad, Pakistan.
Email address.
aqsa_pgmb@yahoo.com
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