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AN ATTEMPT TO CONSERVE WITHANIA SOMNIFERA (L.) DUNAL -
A HIGHLY ESSENTIAL MEDICINAL PLANT, THROUGH IN VITRO CALLUS
CULTURE
JYOTI
RANJAN ROUT*,
SANTI LATA SAHOO
AND RITARANI DAS
Abstract: A
simple effective protocol was developed for conservation and plant
propagation through callus cultures of Withania somnifera
(Ashwagandha). Seed germination percentage reached a maximum value of
64.3% on ½ MS + GA3 0.25 mg/l at third week of
culture. Three different basal media compared for seed germination, MS
was most effective. Out of 25 combinations of growth regulators
evaluated, MS + 1.0 mg/l BA + 1.0 mg/l 2, 4-D found to be
best for callus induction and proliferation regardless to explants.
Among the four different explants tested, In vivo leaf explant
was found most suitable for callus induction, proliferation and fresh
weight gain. The highest callus induction frequency percentage 86.4% was
recorded with In vivo leaf explant whereas, 43.4% in In vitro
leaf explant at day 30 on MS augmented with 1.0 mg/l BA +
1.0 mg/l 2,4-D. Among different growth regulator combinations tested in
augmentation with MS for shoot initiation and elongation, 2.0 mg/l BA +
1.0 mg/l NAA was the best eliciting a maximum of 82.3% shoot
induction with
Biochemistry and
Molecular Biology Laboratory, P.G. Department of
Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India
*Corresponding
author:
E-mail:
routjr@gmail.com
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