SIGNIFICANCE OF DIFFERENT PLANT GROWTH REGULATORS ON THE REGENERATION OF CHRYSANTHEMUM PLANTLETS (DENDRANTHEMA MORIFOLIUM L.) THROUGH SHOOT TIP CULTURE

KASHIF WASEEM^1* MUHAMMAD SALEEM JILANI¹ MUHAMMAD JAFFAR JASKANI², MUHAMMAD SOHAIL KHAN³, MEHWISH KIRAN¹ AND GHAZANFAR ULLAH KHAN

Abstract: Shoot multiplication of Chrysanthemum was achieved from shoot tip explant, using MS (Murashige & Skoog) media supplemented with different concentrations and combinations of plant growth regulators. Different parameters including shoot initiation percentage, average number of shoots per explant, length of shoots (cm), number of leaves per shoot and number of nodes per shoot were studied. Lowest concentration (0.5 mg/L) of naphthalene acetic acid (NAA) excelled all the other concentrations in almost all the parameter studied when used alone. Maximum shoot initiation (80.00%), shoot per explants (3.2), length of shoots (3.4 cm), number of leaves (9.5) and nodes (4.5) were recorded in MS media showed supplemented with 0.5 mg/L NAA. Intermediate concentration (1.0 mg/L) of benzyle adenine purine (BAP) its superiority over all the other BAP concentrations used, when used alone. MS medium fortified with 1.0 mg/L BAP had produced maximum shoot initiation (93.3%), shoot per explant (4.1), length of shoots (5.0 cm) number of leaves (11.0) and nodes (5.5).  Similarly, when the combination of different concentrations of NAA and BAP were used, significant results regarding intermediate concentration of regeneration of chrysanthemum plantlets were achieved. MS media supplemented with lower concentrations of NAA (0.5 and 1.0 mg/L) and BAP (1.0 and 1.5 mg/L) showed better results as compared to other concentrations and combinations. Satisfactory rooting response including days to root emergence (5.0), root initiation percentage (100%), roots per plantlet (14.3) and root length (9.0 cm) was obtained in half strength MS media supplemented with 0.2 mg/L Indole butyric acid (IBA).