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POTENTIAL RAPD
MARKERS FOR POPULATION STUDIES IN TREE LEGUMES
S. MICHAEL GOMEZ1*,
T. RAMASUBRAMANIAN2 AND S. MOHANKUMAR3
Abstract: RAPDs were
quite efficient in bringing out the diversity at DNA level among
non-edible legumes viz.,
Acacia
nilotica, Adenanthera pavonina, Prosopis juliflora, Pithecolobium dulce,
Clitoria ternatea
and Pongamia pinnata.
The RAPD primer
index reveals the information content of the RAPD primer per se.
Of the 82 primers tested, OPE 8, OPI 6, OPL 2, OPL 16, OPI 18, OPI 13,
OPI 14, OPP 1, OPE 20 and OPI 4 with comparatively higher primer index
were more informative and can be used for further DNA finger printing
and population studies in tree legumes. CTAB protocol was found to be
superior in isolating genomic DNA of good quality. The 260/280 ratios
varied between 1.70 and 2.09. Though the genomic DNA isolated by
potassium acetate method was found to be intact in 0.8% agarose gel, the
yield was significantly lower than the modified CTAB method.
1Agri Life Research, Texas A&M System, Lubbock, Texas-79403 USA
2Division of Crop Protection Central Research Institute for
Jute and Allied Fibres Indian Council of
Agricultural Research, Barrackpore, Kolkata-700 120
3Department of Plant Molecular Biology and Biotechnology Centre for Plant
Molecular Biology, Tamil Nadu
Agricultural University Coimbatore – 641 003, Tamil Nadu, India
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