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INDUCED GENETIC VARIABILITY IN CHICKPEA (CICER ARIETINUM L.) III. FREQUENCY OF MORPHOLOGICAL MUTATIONS
TARIQ MAHMUD SHAH1*,
BABAR MANZOOR ATTA1, JAVED IQBAL MIRZA2 AND MUHAMMAD AHSANUL
HAQ1
Abstract:
A comparative study for frequency of morphological mutations
induced by physical (gamma rays) and chemical (EMS) mutagens in M2
population was conducted in two desi (Pb2000, C44), one each of kabuli
(Pb1) and desi x kabuli introgression genotypes (CH 40/91) of chickpea.
In M2 population, about 63 different types of morphological
mutations in different parts of the plants, such as branching pattern,
stem structure, growth habit, foliage type, plant height, pod and seed
size, foliage color, flowering behavior and maturity was observed. The
highest frequency of morphological mutations was observed in 300 Gy
gamma irradiation (4.11%) of CH40/91 followed by 0.4% EMS (2.08%) of
desi genotype C44. The overall frequency of mutations in physical
mutagen was high in CH40/91 (2.69%) followed by Pb2000 (1.38%), C44
(1.02%) and Pb-1 (0.27%). The overall frequency of morphological
mutations in chemical mutagen was very low in Pb2000 (0.45%) followed by
Pb-1 (0.78%), C44 (1.53%) and CH40/91 (1.90%) as compared to physical
mutagens. Overall frequency of morphological mutations in M2
population also indicated maximum mutability (2.30%) in introgression
genotype (CH40/91) followed by desi genotype C44 (1.28%) and Pb2000
(0.91%), whereas kabuli type had lowest mutation rate (0.52%). The
significant higher morphological as well as chlorophyll frequency of
CH40/91 as compared to other three genotypes could be due to the diverse
genetic nature. The gamma rays and EMS treatments differ in their
mutation spectra/frequency.
1Nuclear
Institute for Agriculture and Biology (NIAB), Jhang Road, Faisalabad,
Pakistan
2Institute
of Pure and Applied Biology, Bahauddin Zakariya University, Multan,
Pakistan
*Email:
Shahge266@gmail.com
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