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Interspecific
Variation of Total Seed Protein in wild Rice Germplasm
using SDS-PAGE
Syed Mehar Ali Shah1,
Hidayat-ur-Rahman1, Fida Muhammad Abbasi2, Malik
Ashiq Rabbani3, Ijaz ahmad khan4, Zabta Khan
Shinwari5 and Zahir Shah6
Abstract:
Variation in seed
protein of 14 wild rice species (Oryza spp.) along with
cultivated rice species (O. sativa) was studied using sodium
dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) to assess
genetic diversity in the rice germplasm. SDS bands were scored as
present (1) or absent (0) for protein sample of each genotype. On the
basis of cluster analysis, four clusters were identified at a similarity
level of 0.85. O. nivara, O. rufipogon and O. sativa
with AA genomes constituted the first cluster. The second cluster
comprised O. punctata of BB genome and wild rice species of CC
genome i.e., O. rhizomatis and O. officinalis.
However, it also contained O. barthii and O.
glumaepatula of AA genome. O. australiensis with EE genome,
and O. latifolia, O. alta and O. grandiglumis
having CCDD genomes comprised the third cluster. The fourth cluster
consisted of wild rice species, O. brachyantha with EE genome
along with two other wild rice species, O. longistaminata and
O. meridionalis of AA genome. Overall, on the basis of total seed
protein, the grouping pattern of rice genotypes was mostly compatible
with their genome status. The results of the present work depicted
considerable interspecific genetic variation in the investigated
germplasm for total seed protein. Moreover, the results obtained in this
study also suggest that analysis of seed protein can also provide a
better understanding of genetic affinity of the germplasm.
1Department of Plant Breeding & Genetics, KP Agricultural University,
Peshawar, Pakistan
2Department of Genetics, Hazara University, Manshera, Pakistan
3Institute of Agri-Biotechnology & Genetic Resources, NARC, Islamabad,
Pakistan
4Department of Weed Science, KP Agricultural University, Peshawar,
Pakistan
5Department of Biotechnology, Quaid-i-Azam University, Islamabad,
Pakistan.
6Department of Soil and Environmental Sciences, KP Agricultural
University, Peshawar, Pakistan
*Corresponding
author E-mail:
mehrpbg@gmail.com
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