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Genetic Diversity of
Sesame (Sesamum indicum L.) Germplasm from Pakistan using RAPD
Markers
Fazal Akbar1,
M. Ashiq Rabbani2,
M. SHAHID Masood2
and Zabta K. Shinwari3*
Abstract:
Genetic diversity among
20 sesame (Sesamum indicum L.) accessions was examined at DNA
level by means of random amplified polymorphic DNA (RAPD) analysis. Ten
primers used produced a total of 93 RAPD fragments, of which 70 (75%)
were polymorphic. Each primer generated 5 to 17 amplified fragments with
an average of 9.3 bands per primer. Based on pair-wise comparisons of
RAPD amplification products, Nei and Li’s similarity coefficients were
computed to assess the associations among the accessions. Pair-wise
similarity indices varied from 0.65 to 0.91. A UPGMA cluster analysis
based on these genetic similarities located most of the accessions far
apart from one another, showing a high level of polymorphism.
Genetically, all the genotypes were classified into two major groups and
six subgroups or clusters. A single accession (22243) was relatively
distinct from rest of the accessions and created independent cluster. In
conclusion, even with the use of a limited set of primers, RAPD
technique revealed a high level of genetic variation among sesame
accessions collected from diverse ecologies of Pakistan. This high level
of genetic diversity among the genotypes suggested that RAPD technique
is valuable for sesame systematic, and can be helpful for the upholding
of germplasm banks and the competent choice of parents in breeding
programs.
1Department
of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
2Institute of Agri-Biotechnology & Genetic Resources, NARC, Islamabad,
Pakistan
3Department
of Biotechnology, Quaid-i-Azam
University, Islamabad, Pakistan
*Corresponding author’s
E-mail:
shinwari@qau.edu.pk
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