|
|
|
DETECTION OF SODIUM AZIDE-INDUCED MUTAGENICITY IN THE REGENERATED SHOOTS
OF ARTEMISIA ANNUA L., USING INTERNAL TRANSCRIBED
SPACER (ITS) SEQUENCES OF nrDNA
FAHAD AL-QURAINY1, FAHAD M. AL-HEMAID1, SALIM
KHAN*1, M. AJMAL ALI1,
M. TARROUM1 AND M. ASHRAF1,2
Abstract:
Sodium azide (NaN3) is a well known chemical mutagen which
can effectively cause point mutation in plant genome. The mutagenicity
by this potential mutagen was assessed in the regenerated mutant shoots
of Artemisia annua using internal transcribed spacer (ITS)
sequences of nrDNA. Insertions and/or deletions were detected in
nrDNA-ITS sequences of all mutant shoots and compared with control ones
using the ClustalX program. The regenerated shoots TS1 and
TS2 had deleted bases, whereas TS3, TS4 and TS5 had insertions, because
NaN3 replaced the cytosine (C) by thymine (T) (C→T) (shoots;
TS1and TS4) and thymine (T) replaced by guanine (G) (T→G) (shoot; TS5),
respectively. Artemisinin content was also measured in the regenerated
six-week-old shoots of A. annua. All regenerated shoots had
enhanced level of this compound as compared to that in the controls,
being highest in the regenerated shoot TS3.
1Department of Botany and Microbiology, College of Science, King Saud
University, Riyadh,
Kingdom of Saudi Arabia, 11451.
2Department of Botany, University of Agriculture, Faisalabad, Pakistan
|
|
|